印度尼西亚雅加达,用ELISA和LCMS方法检测轮班护士褪黑激素水平

摘要

Yosephin Sri Sutanti, Muchtaruddin Mansyur, Nurhadi Ibrahim, Martina Wiwie S. Nasrun, Indah sui Widyahening, Dewi Irawati, Yusra Yusra, Rimawati Tedjasukmana

护士的压力很大,因为这直接关系到在轮班工作中应付各种各样的病人。到目前为止,压力案例的发现是基于一个问卷,其中的效度和相关性是相当好的,虽然主观,如感知压力得分(PSS)问卷。本研究旨在通过测量褪黑激素水平,探讨轮班护士压力的客观指标。方法:该研究于2019年12月至2020年6月进行。调查对象为印度尼西亚雅加达某公立医院轮班护士和非轮班护士,通过连续抽样获得。每组由40人组成。褪黑激素测量两次,分别在换班组最后一班夜班午夜(第二晚)(工作前)和第二天早上8点(工作后)。非轮班组在工作日工作前的上午8点和下班后的下午4点进行。Mea采用酶联免疫吸附试验(ELISA)和液相色谱-质谱try (LCMS)。结果:倒班组有mod压力水平的护士比例(30%)高于非倒班组(25%)。 There was a significant difference in the mean pre-work melatonin levels, measured with ELISA, between the shift and the non-shift group (51.53 pg/ml vs 17.07 pg/ml, P = ≤ 0,001), as well as the postwork examination (24.30 pg/ml vs 10.84 pg/ml, P = ≤ 0,001). We acquired a new reference for mean melatonin levels at 04.00 pm of 10.48 pg/mL (2.15-38.30 pg/mL) using ELISA and 38.13 pg/mL (5.00-230.00 pg/ mL) using LCMS in non-shift nurses. In addition, a new reference for melatonin levels at 12.00 pm was 51.53 pg/mL (0.80-135.00 pg/mL) using ELISA and 52.35 pg/ mL (10.00-170.00 pg/mL) using LCMS in shift nurses. Discussion: This study showed differences in the results of measuring melatonin levels using ELISA and LCMS, which will be a good starting point for further research with a larger number of respondents. In addition, it is interesting to observe that a decrease in the mean level of melatonin from pre-work to post-work was found in both the nightshift and dayshift groups, using ELISA and LCMS.

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